期刊
AGING-US
卷 13, 期 5, 页码 7067-7083出版社
IMPACT JOURNALS LLC
DOI: 10.18632/aging.202560
关键词
GIT1; myelin debris; autophagy; angiogenesis; spinal cord injury
资金
- National Natural Science Foundation of China [81772351, 81772352, 81520108018, 81902211]
- Jiangsu Committee of Science and Technology?Social Development Plan [BE2017755]
- High Level Innovation Team [JX102GSP201727]
- Jiangsu Health Commission Foundation [H2018023]
- development Foundation from Wuxi Department of Science and Technology [N20192001]
- Jiangsu Natural Science Foundation [BK20191061, BK20191144]
The study demonstrates the crucial role of GIT1 in promoting MECs clearance of myelin debris and stimulating angiogenesis, making it a promising target for accelerating SCI recovery.
The clearance of myelin debris is a critical step in the functional recovery following spinal cord injury (SCI). As phagocytes do, microvascular endothelial cells (MECs) participate in myelin debris clearance at the injury site within one week. Our group has verified that G protein-coupled receptor kinase 2 interacting protein-1 (GIT1) is essential in autophagy and angiogenesis, both of which are tightly related to the uptake and degradation of myelin debris by MECs. Here, we analyzed the performance and mechanism of GIT1 in myelin debris clearance after SCI. The SCI contusion model was established and in vitro MECs were treated with myelin debris. Better recovery from traumatic SCI was observed in the GIT1 WT mice than in the GIT1 KO mice. More importantly, we found that GIT1 prompted MECs to clear myelin debris and further enhanced MECs angiogenesis in vivo and in vitro. Mechanistically, GIT1-mediated autophagy contributed to the clearance of myelin debris by MECs. In this study, we demonstrated that GIT1 may prompt MECs to clear myelin debris via autophagy and further stimulate MECs angiogenesis via upregulating VEGF. Our results indicate that GIT1 may serve as a promising target for accelerating myelin debris clearance and improving SCI recovery.
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