期刊
AGING CELL
卷 20, 期 3, 页码 -出版社
WILEY
DOI: 10.1111/acel.13334
关键词
ageing; astrocyte; astrocytic complexity; astrocytic cradle; glutamate uptake; K+ buffering; perisynaptic astrocytic processes
资金
- Russian Science Foundation [20-14-00241]
- Russian Science Foundation [20-14-00241] Funding Source: Russian Science Foundation
This study found a significant reduction in the number and length of astrocytic processes, astrocytic territorial domains, and astrocyte-to-astrocyte coupling in the aged brain. Physiological deficits in K+ and glutamate clearance, as well as spatiotemporal reorganisation of Ca2+ events, were observed in old astrocytes, which paralleled impaired synaptic long-term potentiation (LTP) in hippocampal CA1 in old mice. These findings may explain the astroglial mechanisms of age-dependent decline in learning and memory.
Little is known about age-dependent changes in structure and function of astrocytes and of the impact of these on the cognitive decline in the senescent brain. The prevalent view on the age-dependent increase in reactive astrogliosis and astrocytic hypertrophy requires scrutiny and detailed analysis. Using two-photon microscopy in conjunction with 3D reconstruction, Sholl and volume fraction analysis, we demonstrate a significant reduction in the number and the length of astrocytic processes, in astrocytic territorial domains and in astrocyte-to-astrocyte coupling in the aged brain. Probing physiology of astrocytes with patch clamp, and Ca2+ imaging revealed deficits in K+ and glutamate clearance and spatiotemporal reorganisation of Ca2+ events in old astrocytes. These changes paralleled impaired synaptic long-term potentiation (LTP) in hippocampal CA1 in old mice. Our findings may explain the astroglial mechanisms of age-dependent decline in learning and memory.
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