期刊
ADVANCED FUNCTIONAL MATERIALS
卷 31, 期 21, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.202010169
关键词
biomedical devices; cell encapsulation; hydrogel coatings; immune isolation; tissue engineering
类别
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Korean Ministry of Science, ICT, and Future Planning [2015R1A5A2009124, 2020R1C1C1004733]
- National Research Foundation of Korea [2020R1C1C1004733, 5120200313763] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The STIG method allows for universal coating of biomedical devices with different scales, shapes, and components, achieving precise, complete, and individual cell encapsulation. The thickness of the hydrogel layers can be easily controlled by adjusting the gelation time and ion release rate. This low-cost and easy-to-implement method has the potential to bring significant advances in various fields.
Hydrogel coatings have been proposed as a promising strategy to improve the biocompatibility of therapeutic cells and biomedical devices. However, developed coating methods are only applicable for simple geometries, typical sizes, and limited substrates. In addition, its applications in therapeutic cell encapsulation are hampered by inadequate construction of the hydrogel capsules such as off-center encapsulation, immense volume, and lack of control over the thickness of capsules. Here, a method called surface-triggered in situ gelation (STIG) for universal hydrogel coating of multiscale objects ranging from single cells to mini-organs to biomedical devices with arbitrary shapes and heterogeneous components is reported. By covering cells or devices with calcium carbonate particles, progressive propagation of alginate hydrogel from their surface under the stimulation of GDL is achieved. The thickness of the hydrogel layers can be easily controlled from several micrometers to hundreds of micrometers by adjusting the gelation time and the release rate of calcium ions. Importantly, STIG facilitates accurate, complete, and individual cell encapsulation, which potentially overcomes the pitfalls of conventional strategies. It is further proven that the low-cost and facile method can potentially lead to advances in different fields by rendering precisely controlled microscale alginate layers on a wide variety of biomedical substrates.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据