4.8 Article

Matrix stiffening by self-mineralizable guided bone regeneration

期刊

ACTA BIOMATERIALIA
卷 125, 期 -, 页码 112-125

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2021.02.012

关键词

Collagen membranes; Guided bone regeneration; In-situ mineralization; Matrix stiffness mechanotransduction

资金

  1. National Nature Science Foundation of China [81722015, 81870805, 81870787, 81671012, 81720108011]
  2. Shaanxi Key Scientific and Technological Innovation Team [2020TD-033]
  3. Youth Innovation Team of Shaanxi Universities

向作者/读者索取更多资源

This study developed a self-mineralization strategy by covalently conjugating polyacrylic acid on Bio-Gide? membranes, enhancing mineralization and promoting bone regeneration. Experimental results showed that HBG underwent progressive mineralization in vivo, increased stiffness, and stimulated osteogenic differentiation of MSCs to expedite in-situ bone regeneration.
Collagen membranes produced in vitro with different degrees of intrafibrillar mineralization are poten-tially useful for guided bone regeneration (GBR). However, highly-mineralized collagen membranes are brittle and difficult for clinical manipulation. The present study aimed at developing an intrafibrillar self-mineralization strategy for GBR membrane by covalently conjugating high-molecular weight polyacrylic acid (HPAA) on Bio-Gide? membranes (BG). The properties of the self-mineralizable membranes (HBG) and their potential to induce bone regeneration were investigated. The HBG underwent the progressive intrafibrillar mineralization as well as the increase in stiffness after immersed in supersaturated calcium phosphate solution, osteogenic medium, or after being implanted into a murine calvarial bone defect. The HBG promoted in-situ bone regeneration via stimulating osteogenic differentiation of mesenchymal stro-mal cells (MSCs). Hippo signaling was inhibited when MSCs were cultured on the self-mineralized HBG, and in HBG-promoted MSC osteogenesis during in-situ bone regeneration. This resulted in translocation of the transcription co-activators Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) into the nucleus to induce transcription of genes promoting osteogenic differentiation of MSCs. Taken together, these findings indicated that HBG possessed the ability to self-mineralize in situ via intrafibrillar mineralization. The increase in stiffness of the extracellular matrix expedited in-situ bone regeneration by inactivating the Hippo-YAP/TAZ signaling cascade.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据