期刊
ACS NANO
卷 15, 期 2, 页码 3284-3294出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.0c10114
关键词
DNA origami; nanostructures; melting; thermodynamic modeling; hybridization; self-assembly yield
Understanding the folding process of DNA origami is crucial for the broader application of nucleic acid nanofabrication technology. By real-time probing the unit operation of origami assembly, a previously unobserved blocked state was elucidated, which acts as a limit on yield for single folds and may pose a barrier in whole origami assembly.
Understanding the folding process of DNA origami is a critical stepping stone to the broader implementation of nucleic acid nanofabrication technology but is notably nontrivial. Origami are formed by several hundred cooperative hybridization events-folds-between spatially separate domains of a scaffold, derived from a viral genome, and oligomeric staples. Individual events are difficult to detect. Here, we present a real-time probe of the unit operation of origami assembly, a single fold, across the scaffold as a function of hybridization domain separation-fold distance-and staple/scaffold ratio. This approach to the folding problem elucidates a predicted but previously unobserved blocked state that acts as a limit on yield for single folds, which may manifest as a barrier in whole origami assembly.
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