期刊
SEPARATIONS
卷 7, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/separations7040072
关键词
carminic acid; microchip electrophoresis; lab-on-a-chip; photometric detection; foodstuffs; pharmaceuticals
资金
- Slovak Research and Development Agency [APVV-17-0318, APVV-17-0373]
- Slovak Grant Agency for Science [VEGA 1/0787/18]
- Comenius University [UK/19/2020]
This paper presents a novel miniaturized analytical method for the determination of carminic acid, a natural red food dye, in complex food and pharmaceutical matrices by microchip electrophoresis (MCE) with photometric detection. MCE has become a very attractive microscale separation technique because it offers high-speed, high-throughput, small sample injection volume and low reagents consumption. Fast determination of carminic acid in less than 5 min was achieved on a poly(methyl methacrylate) microchip in anionic separation mode at pH 6. Photometric detector based on light-emitting diode technology was set to a wavelength of 490 nm. Using a sample injection volume of 900 nL, a limit of detection of 69 nmol L-1 was achieved. A wide linear dynamic range over four orders of magnitude (from nmol L-1 to mmol L-1) was observed for peak area. Developed method provided favorable intra- and inter-day repeatability of the migration time (up to 2.5% RSD), as well as the repeatability of the peak area (less than 1.9% RSD), regardless of the sample type. The content of carminic acid was determined in various foodstuffs and pharmaceuticals, such as candies, saffron, non-alcoholic drink, and sore throat lozenges with good recoveries (92.5-104.0%).
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