期刊
ISCIENCE
卷 23, 期 12, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.isci.2020.101865
关键词
-
资金
- National Institutes of Health (NIH) [R01 NS39600, NS086082]
- BRAIN Initiative Cell Census Network (BICCN) [U01 MH114829]
Microtubules (MTs) and F-actin (F-act) have long been recognized as key regulators of dendritic morphology. Nevertheless, precisely ascertaining their distinct influences on dendritic trees have been hampered until now by the lack of direct, arbor-wide cytoskeletal quantification. We pair live confocal imaging of fluorescently labeled dendritic arborization (da) neurons in Drosophila larvae with completemulti-signal neural tracing to separatelymeasureMTs and F-act. We demonstrate that dendritic arbor length is highly interrelated with local MT quantity, whereas local F-act enrichment is associated with dendritic branching. Computational simulation of arbor structure solely constrained by experimentally observed subcellular distributions of these cytoskeletal components generated synthetic morphological and molecular patterns statistically equivalent to those of real da neurons, corroborating the efficacy of local MT and F-act in describing dendritic architecture. The analysis and modeling outcomes hold true for the simplest (class I), most complex (class IV), and genetically altered (Formin3 overexpression) da neuron types.
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