期刊
JOURNAL OF FUNGI
卷 6, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/jof6040369
关键词
Olea europaea; Trichoderma; secondary metabolites; defense-related genes; real-time RT-PCR; LC– MS Q-TOF; phenolic compounds; oleuropein; Fusicladium oleagineum
资金
- MIURPON [Linfa 03PE_00026_1, Marea 03PE_00106]
- POR FESR CAMPANIA [CUP B63D18000270007]
- MISE [F/050421/01-03/X32]
- PRIN [PROSPECT 2017JLN833]
- Regione Veneto PSR [DIVINE 3589659]
Biocontrol fungal strains of the genus Trichoderma can antagonize numerous plant pathogens and promote plant growth using different mechanisms of action, including the production of secondary metabolites (SMs). In this work we analyzed the effects of repeated applications of selected Trichoderma strains or SMs on young olive trees on the stimulation of plant growth and on the development of olive leaf spot disease caused by Fusicladium oleagineum. In addition, metabolomic analyses and gene expression profiles of olive leaves were carried out by LC-MS Q-TOF and real-time RT-PCR, respectively. A total of 104 phenolic compounds were detected from olive leave extracts and 20 were putatively identified. Targeted and untargeted approaches revealed significant differences in both the number and type of phenolic compounds accumulated in olive leaves after Trichoderma applications, as compared to water-treated plants. Different secoiridoids were less abundant in treated plants than in controls, while the accumulation of flavonoids (including luteolin and apigenin derivatives) increased following the application of specific Trichoderma strain. The induction of defense-related genes, and of genes involved in the synthesis of the secoiridoid oleuropein, was also analyzed and revealed a significant variation of gene expression according to the strain or metabolite applied.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据