期刊
JOURNAL OF FUNGI
卷 7, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/jof7010019
关键词
monoclonal antibody; ribotoxin; Asp f I; Aspergillus fumigatus; lateral-flow device; urine
资金
- ISCA Diagnostics Ltd. by Innovate UK [105440]
- MRC [MR/N006364/2] Funding Source: UKRI
This study introduces the development of a monoclonal antibody PD7 specific to A. fumigatus and related species as a potential tool for detecting invasive pulmonary aspergillosis. The antibody has a detection limit of about 15 ng Asp f I/mL urine. Preliminary evidence suggests the antibody may be useful for patients with acute lymphoid leukaemia.
Invasive pulmonary aspergillosis (IPA) caused by Aspergillus fumigatus is a life-threatening lung disease of immunocompromised patients. Diagnosis currently relies on non-specific chest CT, culture of the fungus from invasive lung biopsy, and detection of the cell wall carbohydrate galactomannan (GM) in serum or in BAL fluids recovered during invasive bronchoscopy. Urine provides an ideal bodily fluid for the non-invasive detection of pathogen biomarkers, with current urine-based immunodiagnostics for IPA focused on GM. Surrogate protein biomarkers might serve to improve disease detection. Here, we report the development of a monoclonal antibody (mAb), PD7, which is specific to A. fumigatus and related species in the section Fumigati, and which binds to its 18 kDa ribotoxin Asp f I. Using PD7, we show that the protein is secreted during hyphal development, and so represents an ideal candidate for detecting invasive growth. We have developed a lateral-flow device (Afu-LFD(R)) incorporating the mAb which has a limit of detection of similar to 15 ng Asp f I/mL urine. Preliminary evidence of the test's diagnostic potential is demonstrated with urine from a patient with acute lymphoid leukaemia with probable IPA. The Afu-LFD(R) therefore provides a potential novel opportunity for non-invasive urine-based detection of IPA caused by A. fumigatus.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据