期刊
BIOACTIVE MATERIALS
卷 6, 期 1, 页码 137-145出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2020.07.007
关键词
Cryogenic 3D printing; Dual-delivery; Osteogenesis; Angiogenesis; Bone regeneration
资金
- Dongguan University of Technology [KCYCXPT201603, TDYB2019003]
- Department of Education of Guangdong Province, China [2016KQNCX168]
- Natural Science Foundation of Guangdong Province, China [2018A0303130019]
- Natural Science Foundation of China [81772428, 81801859]
- Shenzhen Basic Research Project [JCYJ20180305125254860]
- Guangxi Science and Technology Program, China [2018GXNSFAA138074, 2018GXNSFAA294116]
- Scientific Research Project of High-Level Talents in the affiliated Hospital of Youjiang Medical College for Nationalities, China [R20196306]
3D printing is increasingly used to create advanced bone tissue engineering scaffolds with biomimetic structures and matched mechanical strengths for improved bone regeneration. Customized scaffolds were produced using cryogenic 3D printing of beta-tricalcium phosphate and osteogenic peptide inks, and coated with angiogenic peptide hydrogel to enhance vascularization. The scaffolds had a hierarchically porous structure similar to human cancellous bone and showed high viability for both endothelial cells and mesenchymal stem cells. Improved in vitro migration and angiogenesis were observed for scaffolds with angiogenic peptide, while enhanced osteogenic differentiation was seen in scaffolds containing osteogenic peptide. In vivo, scaffolds with both peptides showed increased angiogenesis and new bone formation.
Three-dimensional (3D) printing has been increasingly employed to produce advanced bone tissue engineering scaffolds with biomimetic structures and matched mechanical strengths, in order to induce improved bone regeneration in defects with a critical size. Given that the successful bone regeneration requires both excellent osteogenesis and vascularization, endowing scaffolds with both strong bone forming ability and favorable angiogenic potential would be highly desirable to induce improved bone regeneration with required vascularization. In this investigation, customized bone tissue engineering scaffolds with balanced osteoconductivity/osteoinductivity were produced via cryogenic 3D printing of beta-tricalcium phosphate and osteogenic peptide (OP) containing water/poly(lactic-co-glycolic acid)/dichloromethane emulsion inks. The fabricated scaffolds had a hierarchically porous structure and were mechanically comparable to human cancellous bone. Angiogenic peptide (AP) containing collagen I hydrogel was then coated on scaffold surface to further provide scaffolds with angiogenic capability. A sequential release with a quick AP release and a slow but sustained OP release was obtained for the scaffolds. Both rat endothelial cells (ECs) and rat bone marrow derived mesenchymal stem cells (MSCs) showed high viability on scaffolds. Improved in vitro migration and angiogenesis of ECs were obtained for scaffolds delivered with AP while enhanced osteogenic differentiation was observed in scaffolds containing OP. The in vivo results showed that, toward scaffolds containing both AP and OP, the quick release of AP induced obvious angiogenesis in vivo, while the sustained OP release significantly improved the new bone formation. This study provides a facile method to produce dual-delivery scaffolds to achieve multiple functions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据