期刊
BIOACTIVE MATERIALS
卷 6, 期 1, 页码 169-178出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2020.07.016
关键词
Hydroxyapatite (HA); Ceramic microfluidic chip; Drug screening; Biomimetic; Bone-on-a-chip
资金
- Shenzhen Science and Technology Program [JCYJ20170815153105076, GJHZ20180411143347603]
- National Natural Science Foundation of China [31770107, 21874116]
- Science and Technology Program of Guangdong Province [2019B010941002, 2017B090911008]
- Guangdong Natural Science Funds for Distinguished Young Scholars [2016A030306018]
- Science and Technology Program of Guangzhou [201804020060, 202007020002]
- Outstanding Scholar Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory [2018GZR110102001]
In this study, a microfluidic chip using HA as a component was developed to create a highly bionic bone environment, which was found to be more conducive to cell proliferation and osteogenic differentiation compared to PDMS. The chip successfully generated a concentration gradient of model drug and determined the drug's IC50, showing great potential in high-throughput bone-related drug screening and research.
Bone is an important part of the human body structure and plays a vital role in human health. A microfluidic chip that can simulate the structure and function of bone will provide a platform for bone-related biomedical research. Hydroxyapatite (HA), a bioactive ceramic material, has a similar structure and composition to bone mineralization products. In this study, we used HA as a microfluidic chip component to provide a highly bionic bone environment. HA substrates with different microchannel structures were printed by using ceramic stereolithography (SLA) technology, and the minimum trench width was 50 mu m. The HA substrate with microchannels was sealed by a thin polydimethylsiloxane (PDMS) layer to make a HA-PDMS microfluidic chip. Cell culture experiments demonstrated that compared with PDMS, HA was more conducive to the proliferation and osteogenic differentiation of the human foetal osteoblast cell line (hFOB). In addition, the concentration gradient of the model drug doxorubicin hydrochloride (DOX) was successfully generated on a Christmas tree structure HA-PDMS chip, and the half maximal inhibitory concentration (IC50) of DOX was determined. The findings of this study indicate that the HA-PDMS microfluidic chip has great potential in the field of high-throughput bone-related drug screening and bone-related research.
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