期刊
ELECTROCHIMICA ACTA
卷 209, 期 -, 页码 269-277出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2016.04.144
关键词
DNA-biosensor; Electrochemical; Long-range electron transfer; Microelectrode
资金
- program Investissements d'Avenir
- ANR [ANR-10-LABX-XXX, ANR-10-IDEX-0001-02]
We report on the design of electrochemical DNA-biosensors made from 15, 25 and 50 mm diameter gold ultramicroelectrodes (UMEs) in a two-electrode setup. The biosensors were prepared by chemical adsorption of 22-base thiol-labeled DNA-probes in a self-assembled monolayer configuration onto the surface of the gold UME. Hybridization events were transduced by Fe-III/Fe-II redox process electromediated by DNA-redox intercalated mediators. The optimization of the DNA-biosensors was achieved by using different FeIII/FeII ligands (cyanide, cefadroxil, deferoxamine) and intercalators (methylene blue, proflavine). Optimized biosensors allowed direct and selective amperometric detection in the femtomolar range and of single mismatch within a 35 minutes protocol. (C) 2016 Elsevier Ltd. All rights reserved.
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