期刊
PLANTS-BASEL
卷 10, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/plants10010009
关键词
tomato; TYLCV; resistance gene; marker-assisted selection; gene-pyramiding
资金
- Basic Science Research Program through the National Research Foundation of Korea - Ministry of Education [2017R1A2B4011621]
- Golden Seed Project (Center for Horticultural Seed Development) - Ministry of Agriculture, Food, and Rural Affairs of Korea
- Kyungpook National University Research Fund, 2019
- National Research Foundation of Korea [2017R1A2B4011621] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Tomato yellow leaf curl virus (TYLCV) is a major threat to tomato production worldwide, with resistance genes such as Ty-2 showing potential for enhancing resistance. Gene-pyramiding can improve resistance levels, and breeding lines containing both Ty-1 and Ty-2 demonstrate the strongest resistance to TYLCV in field and artificial infections.
Tomato yellow leaf curl virus (TYLCV) is a disease that is damaging to tomato production worldwide. Resistance to TYLCV has been intensively investigated, and single resistance genes such as Ty-1 have been widely deployed in breeding programs. However, resistance-breaking incidences are frequently reported, and achieving durable resistance against TYLCV in the field is important. In this study, gene-specific markers for Ty-2 and ty-5, and closely-linked markers for Ty-4 were developed and applied to distinguish TYLCV resistance in various tomato genotypes. Quantitative infectivity assays using both natural infection in the field and artificial inoculation utilizing infectious TYLCV clones in a growth chamber were optimized and performed to investigate the individual and cumulative levels of resistance. We confirmed that Ty-2 could also be an effective source of resistance for TYLCV control, together with Ty-1. Improvement of resistance as a result of gene-pyramiding was speculated, and breeding lines including both Ty-1 and Ty-2 showed the strongest resistance in both field and artificial infections.
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