期刊
FOODS
卷 10, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/foods10010186
关键词
antioxidants; polyphenol oxidase (PPO) activity; nutraceutical properties; polyphenols
资金
- Italian MIUR PRIN-COFIN Project [2004077043_002]
- USDA National Institute of Food and Agriculture Hatch project [1014919, 00011]
Phenolic compounds in fruit have health benefits and affect post-harvest fruit quality. Enzymatic activity and phenolic concentration play key roles in fruit browning, with different apple cultivars showing varying degrees of browning susceptibility.
Phenolic compounds in fruit provide human health benefits, and they contribute to color, taste, and the preservation of post-harvest fruit quality. Phenolic compounds also serve as modifiers of enzymatic activity, whether inhibition or stimulation. Polyphenol oxidases (PPO) and peroxidases (POD) use phenolic compounds as substrates in oxidative browning. Apple browning leads to flesh color, taste, texture, and flavor degradation, representing a drawback for the variety and its' market appraisal. This study was conducted to investigate the process of browning in 14 apple cultivars throughout post-harvest at three-time points: immediately (T0), one hour (T1), and 24 h (T2) after apples were cut in half. Color parameters L* (lightness), a* (red/green), b* (yellow/blue) were measured, and chroma (Delta C*) and color (Delta E) were calculated to quantify differences between T0x208b;T1 and T1x208b;T2 on the fruit surface. Enzymatic activity (PPO, POD) and phenolic composition were also quantified for each cultivar. 'Granny Smith' and 'Cripps Pink' browned minimally. In contrast, 'Fiesta' and 'Mondial Gala' browned severely, reporting high enzymatic activity and quantified phenolic concentration (QPC). Phenolic compound polymerization appears to play a significant role in enzymatic inhibition. 'Topaz' does not fit the high QPC, PPO, and browning formula, suggesting alternative pathways that contribute to apple browning.
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