期刊
FOODS
卷 10, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/foods10020214
关键词
water status and distribution; microstructure; secondary structure of protein; Atlantic mackerel
资金
- National Natural Science Foundation of China [31972105]
- Central Funds Guiding the Local Science and Technology Development of China [2020JH6/10500002]
This study explored the changes in moisture migration, protein oxidation, microstructure, and physicochemical qualities of Atlantic mackerel during storage at 4 degrees C and 0 degrees C. The storage process led to protein oxidation, changes in protein secondary structure, and alterations in myofibril structure, all of which ultimately affected the quality of the mackerel. Additionally, parameters such as drip loss, TVB-N, TBARS value, and b* value significantly increased with storage time.
Moisture migration, protein oxidation, microstructure, and the physicochemical qualities of Atlantic mackerel during storage at 4 degrees C and 0 degrees C were explored in this study. Three proton components were observed in mackerel muscle using low-field nuclear magnetic resonance relaxation, which were characterized as bound water, immobilized water, and lipid. The relaxation peak of immobilized water shifted to a shorter relaxation time and its intensity decreased with the proceeding of the storage process. T-1 and T-2 weighted images obtained by magnetic resonance imaging showed a slightly continuous decrease in the intensity of water. The significant decrease in sulfhydryl (SH) content and the increase in carbonyl group (CP) content, disulfide bond content, and hydrophobicity revealed the oxidation of protein during storage. The contents of alpha-helixes in proteins decreased while that of random coils increased during storage, which suggested changes in the secondary structure of mackerel protein. The storage process also caused the contraction and fracture of myofibrils, and the granulation of endolysin protein. In addition, the drip loss, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS) value, and b* value increased significantly with the storage time.
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