期刊
PATHOGENS
卷 10, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/pathogens10010009
关键词
Pseudomonas aeruginosa; pyoverdine; macrophages; virulence
类别
资金
- National Institutes of Health [NIGMS R35GM129294, C-1930]
- Cystic Fibrosis Foundation [KANG19H0, KIRIEN20I0]
Pseudomonas aeruginosa is a multidrug-resistant pathogen that causes acute infections in immunocompromised patients, using the siderophore pyoverdine to facilitate infection and virulence. Inhibiting pyoverdine production can reduce pathogen virulence and may be a promising therapeutic target.
Pseudomonas aeruginosa is a multidrug-resistant, opportunistic pathogen that utilizes a wide-range of virulence factors to cause acute, life-threatening infections in immunocompromised patients, especially those in intensive care units. It also causes debilitating chronic infections that shorten lives and worsen the quality of life for cystic fibrosis patients. One of the key virulence factors in P. aeruginosa is the siderophore pyoverdine, which provides the pathogen with iron during infection, regulates the production of secreted toxins, and disrupts host iron and mitochondrial homeostasis. These roles have been characterized in model organisms such as Caenorhabditis elegans and mice. However, an intermediary system, using cell culture to investigate the activity of this siderophore has been absent. In this report, we describe such a system, using murine macrophages treated with pyoverdine. We demonstrate that pyoverdine-rich filtrates from P. aeruginosa exhibit substantial cytotoxicity, and that the inhibition of pyoverdine production (genetic or chemical) is sufficient to mitigate virulence. Furthermore, consistent with previous observations made in C. elegans, pyoverdine translocates into cells and disrupts host mitochondrial homeostasis. Most importantly, we observe a strong correlation between pyoverdine production and virulence in P. aeruginosa clinical isolates, confirming pyoverdine's value as a promising target for therapeutic intervention. This in vitro cell culture model will allow rapid validation of pyoverdine antivirulents in a simple but physiologically relevant manner.
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