期刊
ANNALS OF TRANSLATIONAL MEDICINE
卷 8, 期 24, 页码 -出版社
AME PUBL CO
DOI: 10.21037/atm-20-7008
关键词
Apolipoprotein M (apoM); high-density lipoprotein (HDL); inflammatory effect; peripheral blood mononuclear cell (PBMC); scavenger receptor class B type I
资金
- National Natural Science Foundation of China [81201352, 81370372]
- Jiangsu Provincial Natural Science Foundation of China [BK2012154, BK20130244, BK20151179]
- International Cooperation Item of Changzhou [CZ20150012]
- Changzhou High-Level Medical Talents Training Project [2016ZCLJ002]
Background: Inflammation participates pivotally in the pathogenesis of atherosclerosis. Apolipoprotein M (apoM) is a high-density lipoprotein (HDL)-associated plasma protein that affects HDL metabolism and shows various anti-inflammatory functions in atherosclerosis. In this study, we aim to determine whether apoM is expressed in peripheral blood mononuclear cells (PBMCs) and promoted the anti-inflammatory effect of HDL by combing with scavenger receptor BI (SR-BI). Methods: The expression of apoM in PBMCs is detected by a confocal fluorescence microscope and flow cytometry. The interactions between apoM and SR-BI are detected with co-immunoprecipitation. The multiplexed Luminex xMAP assay detects the inflammatory factors induced by apoM(+) HDL and apoM(-) HDL in inflammatory cell model. Results: ApoM is expressed on CD14(+) monocytes, CD3(+) T cells, and CD19(+) B cells, CD16(+) and CD56(+) NK cells. CD14(+) monocytes have the highest ratio of apoM(+) cells. ApoM(+) HDL, apoM(-) HDL, and recombinant apoM protein could be co-precipitated with SR-BI on the surface of human THP-1 monocytic leukemia cells. In vitro, apoM(+) HDL induces significantly less expression of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and IL-1 beta than apoM(-) HDL. Conclusions: ApoM was expressed on all PBMCs. ApoM interacted with SR-BI on THP-1. ApoM(+) HDL has a more significant anti-inflammatory effect than apoM(-) HDL.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据