期刊
MICROORGANISMS
卷 9, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9020233
关键词
Saccharomyces cerevisiae; carotenoids; copper; zinc; ACE1; ADY2
类别
资金
- Key-Area Research and Development Program of Guangdong Province [2018B020206001]
- Science and Technology Plan Project of Guangdong Province [2016A010105013, 2019B030316017]
Nutritional components significantly impact carotenoid accumulation in engineered Saccharomyces cerevisiae, with copper ions playing a prominent role in increasing carotenoid yield. Transcriptome analysis revealed key genes like ADY2, HES1, and CUP1, and overexpression of ACE1 in YPD media led to a 6.4-fold increase in carotenoid yield. This study provides insights for genetic engineering strategies to enhance carotenoid production in yeast.
We previously constructed a Saccharomyces cerevisiae carotenoid producer BL03-D-4 which produced much more carotenoid in YPM (modified YPD) media than YPD media. In this study, the impacts of nutritional components on carotenoid accumulation of BL03-D-4 were investigated. When using YPM media, the carotenoid yield was increased 10-fold compared to using the YPD media. To elucidate the hidden mechanism, a transcriptome analysis was performed and showed that 464 genes changed significantly in YPM media. Furthermore, inspired by the differential gene expression analysis which indicated that ADY2, HES1, and CUP1 showed the most remarkable changes, we found that the improvement of carotenoid accumulation in YPM media was mainly due to the copper ions, since supplementation of 0.08 mM CuSO4 in YPD media could increase carotenoid yield 9.2-fold. Reverse engineering of target genes was performed and carotenoid yield could be increased 6.4-fold in YPD media through overexpression of ACE1. The present study revealed for the first time the prominent promotion of carotenoid yield by copper ions in engineered S. cerevisiae and provided a new target ACE1 for genetic engineering of S. cerevisiae for the bioproduction of carotenoids.
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