4.7 Article

In Escherichia coli Ammonia Inhibits Cytochrome bo3 But Activates Cytochrome bd-I

期刊

ANTIOXIDANTS
卷 10, 期 1, 页码 -

出版社

MDPI
DOI: 10.3390/antiox10010013

关键词

bacteria; redox enzymes; respiratory oxidases; ammonia; environmental stressor

资金

  1. Russian Foundation for Basic Research [19-04-00094]
  2. Sapienza grant [RP1181643681A66B]

向作者/读者索取更多资源

The interaction between Escherichia coli's redox enzymes, cytochrome bo(3) and cytochrome bd-I, with ammonium sulfate/ammonia at different pH levels was studied. It was found that while cytochrome bo(3) was inhibited by the ligand at pH 8.3, cytochrome bd-I's activity increased with increasing ligand concentration. The effector molecule in both cases appeared to be NH3 rather than NH4+.
Interaction of two redox enzymes of Escherichia coli, cytochrome bo(3) and cytochrome bd-I, with ammonium sulfate/ammonia at pH 7.0 and 8.3 was studied using high-resolution respirometry and absorption spectroscopy. At pH 7.0, the oxygen reductase activity of none of the enzymes is affected by the ligand. At pH 8.3, cytochrome bo(3) is inhibited by the ligand, with 40% maximum inhibition at 100 mM (NH4)(2)SO4. In contrast, the activity of cytochrome bd-I at pH 8.3 increases with increasing the ligand concentration, the largest increase (140%) is observed at 100 mM (NH4)(2)SO4. In both cases, the effector molecule is apparently not NH4+ but NH3. The ligand induces changes in absorption spectra of both oxidized cytochromes at pH 8.3. The magnitude of these changes increases as ammonia concentration is increased, yielding apparent dissociation constants K-dapp of 24.3 +/- 2.7 mM (NH4)(2)SO4 (4.9 +/- 0.5 mM NH3) for the Soret region in cytochrome bo(3), and 35.9 +/- 7.1 and 24.6 +/- 12.4 mM (NH4)(2)SO4 (7.2 +/- 1.4 and 4.9 +/- 2.5 mM NH3) for the Soret and visible regions, respectively, in cytochrome bd-I. Consistently, addition of (NH4)(2)SO4 to cells of the E. coli mutant containing cytochrome bd-I as the only terminal oxidase at pH 8.3 accelerates the O-2 consumption rate, the highest one (140%) being at 27 mM (NH4)(2)SO4. We discuss possible molecular mechanisms and physiological significance of modulation of the enzymatic activities by ammonia present at high concentration in the intestines, a niche occupied by E. coli.

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