期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2020.605096
关键词
biochar; L-histidine; E. coli; biosynthesis; organic compounds
资金
- S&D Program from Bijie Tobacco Company of Guizhou Province Guizhou Tobacco Company [2018520500240066]
- Youth Program of National Natural Science Foundation of China [31801292]
- National Key R&D Program Research and Development of BiocharBased Fertilizer and Microbial Fertilizer [2017YFD0200800, 2017YFD0200802]
- Innovative Talents Promotion Plan of Ministry of Science and Technology [2017RA2211]
- LiaoNing Revitalization Talents Program [XLYC1802094]
- National Natural Science Foundation of China [41807101]
The organic compounds found in biochar act as hormone analogs, stimulating the expression of metabolites by controlling related genes and proteins to promote L-histidine biosynthesis in E. coli. The optimal concentration was determined to be 3%, with 7% being lethal. Docking technology was used to better understand the mechanisms, with GC-MS analysis revealing the connection between three organic molecules and HisG protein in regulating L-histidine biosynthesis.
The organic compounds from biochar play a role of hormone analogs, stimulating the expression of metabolites by controlling related gene and protein. In this experiment, we reported the L-histidine biosysthesis was promoted by biochar treatment in E. coli unlike genetic engineering of the traditional method. The related results indicated the most optimal concentration was found to be 3%, and 7% is the lethal dose. E. coli was inhibited in the high-concentration treatment. On the other hand, docking technology was usually used as drug screening, basing on Lock-and-key model of protein in order to better understand mechanisms. So the organic compounds of biochar from GC-MS analysis that acted as ligands were connected to HisG protein controlling L-histidine biosysthesis in E. coli. The result showed that the three organic molecules interacted with HisG protein by hydrogen bond. So we considered that these three compounds play regulatory roles in L-histidine biosysthesis, and the hisG gene expression fully supports this conclusion.
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