期刊
ELECTROCHEMISTRY COMMUNICATIONS
卷 64, 期 -, 页码 46-50出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2016.01.008
关键词
LIFT; Reaction center; Rhodobacter; Photocurrent; Inhibition; Herbicide
资金
- COST Action [TD1102]
- European Social Fund
- Greek National Research Funding Program Heracleitus II
The development of an amperometric biosensor for herbicide detection, using bacterial reaction centers (RC) as biorecognition element, is presented. RC immobilization on gold screen printed electrodes was achieved by LIFT, a powerful physisorption-based immobilization technique that enhances the intimate contact between the protein and the electrode surface. As a result, stable photocurrents driven by direct electron transfer at the donor side were observed, both in the presence and in the absence of a quinone substrate in solution. The addition of quinone UQ(0) increased the photocurrents, while the UQ(0)-free system showed higher sensitivity to the herbicide terbutryn, a model inhibitor, acting as photocurrent attenuator. In spite of its simple design, the performances achieved by our mediatorless device are comparable or superior to those reported for analogous RC-based photoelectrochemical cells, in terms of both terbutryn sensing and photocurrent generation. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据