期刊
FOOD SCIENCE & NUTRITION
卷 9, 期 1, 页码 469-479出版社
WILEY
DOI: 10.1002/fsn3.2016
关键词
fermented milk; L. helveticus; postacidification; texture; UV mutation
资金
- Natural Science Foundation of Jiangsu Province [BK20170496]
- National Natural Science Foundation of China [31700079]
"By detecting H+-ATPase activity, a weak postacidify mutant of L. helveticus SH2-1 named L. helveticus sh2-5-66 was successfully selected and proven to be genetically stable for multiple generations. Milk fermented with L. helveticus sh2-5-66 demonstrated improved texture, rheological properties, and flavor during storage."
To derive a mutant of L. helveticus SH2-1 with the capacity of weak postacidification and high texturing, first, taking L. delbrueckii frs4-1 and S. thermophilus grx02 as the controls, H+-ATPase activity was demonstrated to be highly related to the postacidification of L. helveticus SH2-1. Then, by detecting H+-ATPase activity, the weak postacidify mutant of L. helveticus SH2-1 (renamed as L. helveticus sh2-5-66) was selected from 80 UV mutants. The pH and acidity of the milk fermented with L. helveticus sh2-5-66 were separately 0.57 pH units higher and 57.1 degrees T lower than that of L. helveticus SH2-1. The acidification of L. helveticus sh2-5-66 was further demonstrated to be genetically stable during 100 generations cultivation. Moreover, the milk fermented with L. helveticus sh2-5-66 showed improvement in textural and rheological properties and flavor during storage which could be further improved by coculture with the commercial starter S. thermophilus st447.
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