期刊
ELECTROANALYSIS
卷 29, 期 4, 页码 1031-1037出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201600697
关键词
Biosensor; laccase; gold nanoparticles; -cyclodextrin; rutin
资金
- FAPESC/CNPq [2807/2012 - PRONEM]
- MCTI/CNPq [442609/2014-0 - Universal]
- CNPq-Brazil
- CNPq
- Central Laboratory of Electron Microscopy, Federal University of Santa Catarina (Florianopolis, SC, Brazil)
This paper describes the synthesis and characterization of gold nanoparticles stabilized in -cyclodextrin (AuNP-CD), which were applied as a platform in the immobilization of laccase (LAC). The AuNP-CD-LAC were used in the construction of a new biosensor for rutin determination by square-wave voltammetry (SWV). Under optimized conditions, the calibration curve showed a linear range for rutin of 0.30 to 2.97 mol L-1, with a limit of detection of 0.17 mol L-1. The biosensor demonstrated satisfactory repeatability and electrode-to-electrode repeatability (with relative standard deviations of 5.6 and 6.0%, respectively) and good stability. The biosensor was successfully applied in the determination of rutin in different pharmaceutical samples.
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