期刊
MICROBIAL BIOTECHNOLOGY
卷 14, 期 3, 页码 967-978出版社
WILEY
DOI: 10.1111/1751-7915.13728
关键词
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资金
- KU Leuven [GOA/15/006]
- Research Foundation - Flanders [12V5219N, 1S56416N, 1S44616N]
The phage proteins known as YIPs interfere with c-di-GMP signaling by directly targeting Pseudomonas diguanylate cyclase YfiN. This results in increased c-di-GMP production, reduced motility, and increased biofilm mass in P. aeruginosa. The phage-based mechanism of metabolic regulation through intracellular signaling interference could inspire the development of new molecules to disrupt biofilm formation in various pathogens.
C-di-GMP is a key signalling molecule which impacts bacterial motility and biofilm formation and is formed by the condensation of two GTP molecules by a diguanylate cyclase. We here describe the identification and characterization of a family of bacteriophage-encoded peptides that directly impact c-di-GMP signalling in Pseudomonas aeruginosa. These phage proteins target Pseudomonas diguanylate cyclase YfiN by direct protein interaction (termed YIPs, YfiN Interacting Peptides). YIPs induce an increase of c-di-GMP production in the host cell, resulting in a decrease in motility and an increase in biofilm mass in P. aeruginosa. A dynamic analysis of the biofilm morphology indicates a denser biofilm structure after induction of the phage protein. This intracellular signalling interference strategy by a lytic phage constitutes an unexplored phage-based mechanism of metabolic regulation and could potentially serve as inspiration for the development of molecules that interfere with biofilm formation in P. aeruginosa and other pathogens.
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