期刊
CELLS
卷 10, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/cells10010145
关键词
allergy; asthma; histamine; IL-33; mast cell; protein A; protein L; superantigen; VEGF-A; VEGF-C
类别
资金
- Regione Campania CISI-Lab Project
- TIMING Project
- Campania Bioscience
- MIUR [M8Y MR8_005]
The study found that immunoglobulin superantigens protein A and protein L can induce rapid release of histamine from HLMCs, while IL-33 does not trigger histamine release from lung mast cells. Prolonged incubation of HLMCs with superantigens and IL-33 leads to the release of VEGF-A and VEGF-C. Preincubation with IL-33 enhances the superantigenic release of histamine, angiogenic and lymphangiogenic factors from HLMCs.
Human lung mast cells (HLMCs) express the high-affinity receptor Fc epsilon RI for IgE and are strategically located in different compartments of human lung, where they play a role in several inflammatory disorders and cancer. Immunoglobulin superantigens (e.g., protein A of Staphylococcus aureus and protein L of Peptostreptococcus magnus) bind to the variable regions of either the heavy (V(H)3) or light chain (kappa) of IgE. IL-33 is a cytokine expressed by epithelial cells that exerts pleiotropic functions in the lung. The present study investigated whether immunoglobulin superantigens protein A and protein L and IL-33 caused the release of inflammatory (histamine), angiogenic (VEGF-A) and lymphangiogenic (VEGF-C) factors from HLMCs. The results show that protein A and protein L induced the rapid (30 min) release of preformed histamine from HLMCs. By contrast, IL-33 did not induce the release of histamine from lung mast cells. Prolonged incubation (12 h) of HLMCs with superantigens and IL-33 induced the release of VEGF-A and VEGF-C. Preincubation with IL-33 potentiated the superantigenic release of histamine, angiogenic and lymphangiogenic factors from HLMCs. Our results suggest that IL-33 might enhance the inflammatory, angiogenic and lymphangiogenic activities of lung mast cells in pulmonary disorders.
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