期刊
JOURNAL OF CLINICAL MEDICINE
卷 10, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/jcm10020285
关键词
lymphocytic colitis; enteroendocrine cells; tryptophan hydroxylase; serotonin; 5-hydroxyindoleacetic acid; budesonide
资金
- Medical University of Lodz [503/6-006-0]
The levels of serotonin and 5-HIAA, as well as the number of EECs, were found to be higher in LC patients compared to healthy controls. Positive correlations were observed between the levels of serotonin and 5-HIAA, serotonin and EEC number, TPH1 mRNA and EEC number, as well as disease severity and 5-HIAA. Budesonide treatment reduced these levels and restored them to values similar to the control group.
Lymphocytic colitis (LC) is a chronic inflammatory disease associated with watery diarrhea, abdominal pain, and colonic intraepithelial lymphocytosis. Serotonin (5-hydroxytryptamine, 5-HT) is reported to increase in certain colon diseases; however, little is known regarding its metabolism in LC. In the present work, the level of 5-HT in serum and the number of enteroendocrine cells (EECs) as well as the expression of the 5-HT rate-limiting enzyme tryptophan hydroxylase 1 (TPH1) in colonic biopsies and urine 5-hydroxyindoeoacetic acid (5-HIAA) were determined in 36 LC patients that were treated with budesonide and 32 healthy controls. The 5-HT serum and 5-HIAA urine levels were measured using ELISA, the EEC number was determined immunohistochemically, and the colonic TPH1 mRNA expression was determined using RT-PCR. The levels of 5-HT and 5-HIAA and the number of EECs were higher in LC patients than in the controls, and positive correlations were observed between the 5-HT and 5-HIAA levels, 5-HT and EEC number, TPH1 mRNA and EEC number, as well as the severity of disease symptoms and 5-HIAA. Budesonide decreased the levels of 5-HT, 5-HIAA, and TPH1 expression and the number of EECs to values that did not differ from those for controls. In conclusion, the serotonin metabolism may be important for LC pathogenesis, and the urinary level of 5-HIAA may be considered as a non-invasive marker of this disease activity.
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