期刊
JOURNAL OF CLINICAL MEDICINE
卷 10, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/jcm10010062
关键词
glaucoma; lamina cribrosa; fibrosis; calcium; PKC alpha; p38-MAPK; p42/44-MAPK
资金
- Irish College of Ophthalmologists/Novartis Research Award 2015/2016
- International Glaucoma Association/Royal College of Ophthalmologists Grant 2017
This study identified abnormalities in multiple calcium signaling pathways (PKC alpha, MAPK, PI3K) in glaucoma LC cells, which may have significant implications for optic nerve head fibrosis and cupping in glaucoma.
The lamina cribrosa (LC) is a key site of fibrotic damage in glaucomatous optic neuropathy and the precise mechanisms of LC change remain unclear. Elevated Ca2+ is a major driver of fibrosis, and therefore intracellular Ca2+ signaling pathways are relevant glaucoma-related mechanisms that need to be studied. Protein kinase C (PKC), mitogen-activated MAPK kinases (p38 and p42/44-MAPK), and the PI3K/mTOR axis are key Ca2+ signal transducers in fibrosis and we therefore investigated their expression and activity in normal and glaucoma cultured LC cells. We show, using Western immune-blotting, that hyposmotic-induced cellular swelling activates PKC alpha, p42/p44, and p38 MAPKs, the activity is transient and biphasic as it peaks between 2 min and 10 min. The expression and activity of PKC alpha, p38 and p42/p44-MAPKs are significantly (p < 0.05) increased in glaucoma LC cells at basal level, and at different time-points after hyposmotic stretch. We also found elevated mRNA expression of mRNA expression of PI3K, IP3R, mTOR, and CaMKII in glaucoma LC cells. This study has identified abnormalities in multiple calcium signaling pathways (PKC alpha, MAPK, PI3K) in glaucoma LC cells, which might have significant functional and therapeutic implications in optic nerve head (ONH) fibrosis and cupping in glaucoma.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据