4.0 Article

Effect of nitrogen-containing bisphosphonates on osteoclasts and osteoclastogenesis: an ultrastructural study

期刊

MICROSCOPY
卷 70, 期 3, 页码 302-307

出版社

OXFORD UNIV PRESS
DOI: 10.1093/jmicro/dfaa073

关键词

alendronate; bisphosphonate; nitrogen-containing bisphosphonate; osteoclast; osteoclastogenesis; transmission; electron microscopy

资金

  1. Ministry of Education, Culture, Sports, Science and Technology of Japan [24592777, 15K11022, 20K18516]
  2. Grants-in-Aid for Scientific Research [15K11022, 20K18516, 24592777] Funding Source: KAKEN

向作者/读者索取更多资源

The study found that alendronate affects mouse osteoclasts by altering cell structure and inducing fusion of osteoclasts to form giant cells.
We have previously indicated that a single injection of alendronate, one of the nitrogen-containing bisphosphonates (NBPs), affects murine hematopoietic processes, such as the shift of erythropoiesis from bone marrow (BM) to spleen, disappearance of BM-resident macrophages, the increase of granulopoiesis in BM and an increase in the number of osteoclasts. NBPs induce apoptosis and the formation of giant osteoclasts in vitro and/or in patients undergoing long-term NBP treatment. Therefore, the time-kinetic effect of NBPs on osteoclasts needs to be clarified. In this study, we examined the effect of alendronate on mouse osteoclasts and osteoclastogenesis. One day after the treatment, osteoclasts lost the clear zone and ruffled borders, and the cell size decreased. After 2 days, the cytoplasm of osteoclasts became electron dense and the nuclei became pyknotic. Some of the cells had fragmented nuclei. After 4 days, osteoclasts had euchromatic nuclei attached to the bone surface. Osteoclasts had no clear zones or ruffled borders. After 7 days, osteoclasts formed giant osteoclasts via the fusion of multinuclear and mononuclear osteoclasts. These results indicate that NBPs affect osteoclasts and osteoclastogenesis via two different mechanisms.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.0
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据