Single-Molecule Forster Resonance Energy Transfer-Based Photosensitizer for Synergistic Photodynamic/Photothermal Therapy

Photosensitizers (PSs) inevitably release a large amount of energy in the form of fluorescence during photodynamic therapy (PDT). However, under the premise of satisfying fluorescence imaging, a large amount of energy is lost, which limits the efficiency of tumor therapy. Accordingly, in this study, we developed a new strategy (BDP-CR) using the single-molecule Forster resonance energy transfer (smFRET) mechanism to transfer part of the fluorescent energy into heat for combined PDT and photothermal therapy (PTT) featuring the 1 + 1 > 2 amplification effect. Under the 671 nm light irradiation, BDP-CR can produce singlet oxygen (O-1(2)) for PDT based on the BDP moiety and also generate hyperthermia to achieve the PTT effect by exciting CR based on the smFRET effect, which effectively kills cancer cells both in vitro and in vivo. This strategy exhibits a broad absorption peak with strong light-harvesting ability, which improves photon utilization for treatment while realizing fluorescence imaging. Of note, owing to the smFRET effect, we achieve a combination treatment outcome at relatively low concentrations and light doses. Thus, we believe that this design concept will provide a new strategy for single-molecule FRET photosensitizers in combination therapy of cancer with potential clinical application prospects.

Automated summary

In this study, a new strategy using the single-molecule Forster resonance energy transfer (smFRET) mechanism was developed to transfer part of the fluorescent energy into heat for combined photodynamic and photothermal therapy. This approach not only improves treatment efficiency but also enables fluorescence imaging. The strategy allows for a combination treatment outcome at relatively low concentrations and light doses.