Biosynthesis and antibacterial activity of MgO-NPs produced from Camellia-sinensis leaves extract

Magnesium oxides nanoparticles (MgO-NPs) were synthesized by a novel technique based on the leaf extract of Camellia sinensis (Green tea). The synthesized nanoparticles were evaluated for antibacterial activity (against both gram-positive and gram-negative pathogens) and therefore can be a suitable therapeutic alternative to the usage of antibiotics. The antibacterial activity of synthesized MgO-NPs is tested against clinical isolates of gram-negative (Escherichia coli, Pseudomonas aeruginosa, Serratia mercescens, Klebsiella pneumoniae) and gram-positive (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes) pathogenic bacteria. Agar well diffusion assay data indicate that MgO-NPs exhibit antibacterial activity at all concentrations tested against both gram-negative and gram-positive pathogenic bacteria, producing zone of inhibition (ZOI) in the range of 9.6 1.1 to 21.0 1.5 mm diameters. The maximum response is observed at 25 mu g ml(-1) concentration of MgO-NPs, producing a zone of inhibition ranging from 15 1.2 mm (E.coli) mm to 21.0 1.5 mm (S. marcescens).

Automated summary

Magnesium oxides nanoparticles (MgO-NPs) synthesized using green tea leaf extract exhibit significant antibacterial activity against both gram-negative and gram-positive pathogens, indicating it as a potential therapeutic alternative to antibiotics. The maximum antibacterial response is observed at a concentration of 25 μg/ml of MgO-NPs, with inhibitory zones ranging from 15±1.2 mm (E.coli) to 21.0±1.5 mm (S. marcescens).