期刊
GIGASCIENCE
卷 10, 期 1, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/gigascience/giaa160
关键词
Macrobrachium nipponense; Chromosome-level genome; Evolutionary analysis; Genome duplication; Candidate sex-related genes
资金
- National Key R&D Program of China [2018YFD0901303]
- Special Scientific Research Funds for Central Non-profit Institutes, CAFS [2020TD36]
- Jiangsu Agricultural Industry Technology System (Macrobrachium nipponense)
- Important New Varieties Selection Project of Jiangsu Province [PZCZ201745]
- China Agriculture Research System-48 (CARS-48)
- Fund of Three Innovations Engineering of Jiangsu Province [D2015-16]
The researchers obtained the first high-quality chromosome-level genome assembly of the oriental river prawn, predicting the chromosome number and a whole-genome duplication event. By integrating genome and transcriptome data, 21 candidate genes related to sex were predicted.
Background: The oriental river prawn, Macrobrachium nipponense, is an economically important shrimp in China. Male prawns have higher commercial value than females because the former grow faster and reach larger sizes. It is therefore important to reveal sex-differentiation and development mechanisms of the oriental river prawn to enable genetic improvement. Results: We sequenced 293.3 Gb of raw Illumina short reads and 405.7 Gb of Pacific Biosciences long reads. The final whole-genome assembly of the Oriental river prawn was similar to 4.5 Gb in size, with predictions of 44,086 protein-coding genes. A total of 49 chromosomes were determined, with an anchor ratio of 94.7% and a scaffold N50 of 86.8 Mb. A whole-genome duplication event was deduced to have happened 109.8 million years ago. By integration of genome and transcriptome data, 21 genes were predicted as sex-related candidate genes. Conclusion: The first high-quality chromosome-level genome assembly of the oriental river prawn was obtained. These genomic data, along with transcriptome sequences, are essential for understanding sex-differentiation and development mechanisms in the oriental river prawn, as well as providing genetic resources for in-depth studies on developmental and evolutionary biology in arthropods.
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