期刊
FRONTIERS IN IMMUNOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2020.579615
关键词
Campylobacter jejuni; colitis; innate lymphoid cells; interleukin-23; Campylobacteriosis; IL-10
类别
资金
- NIH [AI135574, AI111000]
- USDA NIFA [2014-67012-22276]
- Crohn's and Colitis Foundation [294083]
- Max and Minnie Tomerlin Voelcker Fund
- William and Ella Owens Medical Research Foundation
- UT Health San Antonio [NIH-NCI P30 CA054174-20, UL1 TR001120]
IL-23 contributes to intestinal inflammation in C. jejuni infected mice by promoting IL-17 and IFN gamma production by innate lymphoid cells. Reduced levels of pro-inflammatory cytokines and immune cell accumulation in the colon were observed in IL-23(-/-) mice, independent from bacterial clearance. Flow cytometry analysis revealed a decrease in IL-17 and IFN gamma production by group 1 and 3 innate lymphoid cells.
Human pathogen Campylobacter jejuni is a significant risk factor for the development of long-term intestinal dysfunction although the cellular and molecular mechanisms remain scantily defined. IL-23 is an emerging therapeutic target for the treatment of inflammatory intestinal diseases, however its role in C. jejuni-driven intestinal pathology is not fully understood. IL-10 deficient mice represent a robust model to study the pathogenesis of C. jejuni infection because C. jejuni infection of mice lacking IL-10 results in symptoms and pathology that resemble human campylobacteriosis. To determine the role of IL-23 in C. jejuni-driven intestinal inflammation, we studied the disease pathogenesis in IL-23(-/-) mice with inhibited IL-10R alpha signaling. These mice exhibited reduced intestinal pathology independent from bacterial clearance. Further, levels of IFN gamma, IL-17, IL-22, TNF, and IL-6 were reduced and associated with reduced accumulation of neutrophils, monocytes and macrophages in the colon. Flow cytometry analysis revealed reduced production of IL-17 and IFN gamma by group 1 and 3 innate lymphoid cells. Thus, our data suggest that IL-23 contributes to intestinal inflammation in C. jejuni infected mice by promoting IL-17 and IFN gamma production by innate lymphoid cells.
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