期刊
DARU-JOURNAL OF PHARMACEUTICAL SCIENCES
卷 29, 期 1, 页码 23-38出版社
SPRINGER INT PUBL AG
DOI: 10.1007/s40199-020-00378-1
关键词
Alzheimer's disease; Lipoic acid; Chromone; Multifunctional agent; Antioxidant
资金
- National Institute for Medical Research Development (NIMAD) [971370]
Novel chromone-lipoic acid hybrids were prepared and evaluated for their neuroprotection and anticholinesterase activity. Compound 19 showed the most potent butyrylcholinesterase inhibition and significant neuroprotection, making it a hopeful multifunctional agent in the treatment of Alzheimer's disease.
Purpose Alzheimer's disease (AD) is a multifaceted neurodegenerative disease. To target simultaneously multiple pathological processes involved in AD, natural-origin compounds with unique characteristics are promising scaffolds to develop novel multi-target compounds in the treatment of different neurodegenerative disease, especially AD. In this study, novel chromone-lipoic acid hybrids were prepared to find a new multifunctional lead structure for the treatment of AD. Methods Chromone-lipoic acid hybrids were prepared through click reaction and their neuroprotection and anticholinesterase activity were fully evaluated. The anti-amyloid aggregation, antioxidant and metal-chelation activities of the best compound were also investigated by standard methods to find a new multi-functional agent against AD. Results The primary biological screening demonstrated that all compounds had significant neuroprotection activity against H2O2-induced cell damage in PC12 cells. Compound 19 as the most potent butyrylcholinesterase (BuChE) inhibitor (IC50 = 7.55 mu M) having significant neuroprotection activity as level as reference drug was selected for further biological evaluations. Docking and kinetic studies revealed non-competitive mixed-type inhibition of BuChE by compound 19. It could significantly reduce formation of the intracellular reactive oxygen species (ROS) and showed excellent reducing power (85.57 mM Fe+2), comparable with quercetin and lipoic acid. It could also moderately inhibit A beta aggregation and selectively chelate with copper ions in 2:1 M ratio. Conclusion Compound 19 could be considered as a hopeful multifunctional agent for the further development gainst AD owing to the acceptable neuroprotective and anti-BuChE activity, moderate anti-A beta aggregation activity, outstanding antioxidant activity as well as selective copper chelation ability.
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