期刊
BIOENGINEERED
卷 12, 期 1, 页码 196-207出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2020.1860492
关键词
Lung cancer; long non-coding RNA PGM5P4-AS1; microRNA miR-1275/leucine zipper putative tumor suppressor 3; proliferation; metastasis
资金
- Technological Innovation Project of Cooperation Fund in Hebei Provincial Department of Science and Technology and Hebei Medical University [2020TXZH04]
- Key Project of Health Technology Research and Achievement Conversion in Hebei Province [zh2018009]
This study identified a new lncRNA PGM5P4-AS1, which was lower expressed in lung cancer tissues and could inhibit the proliferation, migration, and invasion abilities of lung cancer cells when overexpressed. PGM5P4-AS1 positively regulated the expression of LZTS3 by sponging miR-1275.
It is necessary to explore new molecules for the improvement of precise diagnosis and antitumor therapies in lung cancer. LncRNAs (long non-coding RNAs) play an important role in the regulation of cancer cell malignant behavior and tumor development. In this work, we found that a newly discovered lncRNA, lncRNA PGM5P4-AS1, was lower expressed in lung cancer tissues than adjacent tissues. Then, the lncRNA PGM5P4-AS1 was overexpressed or knocked-down in different lung cancer cells, and its effects on the malignant phenotypes were measured by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, cell cycle assay, wound healing assay, and transwell assay. The results showed that the overexpression of PGM5P4-AS1 inhibited lung cancer cell proliferation, migration, and invasion activities, while these abilities were prominently promoted by the interference of PGM5P4-AS1. Further, the growth of lung cancer tumors in nude mice was also inhibited by PGM5P4-AS1 overexpression. In mechanism, PGM5P4-AS1 has the binding site of miR-1275 and could positively regulate the expression of LZTS3 via sponging miR-1275. In conclusion, PGM5P4-AS1 could be a potential precise diagnosis and therapeutic target biomarker of lung cancer. [GRAPHICS]
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