Morphological Study of Bacillus thuringiensis Crystals and Spores

In this paper, we report a morphological study of the crystals and spores of different shapes synthesized by seven different strains of Bacillus thuringiensis. Crystals and spores were separated after 48 h of culture on T3 agar medium and imaged under a scanning electron microscope (SEM). Sizes of the crystals and spores were determined using Image J software. The results showed that crystal and spore sizes were normally distributed. In addition, the volumes and aspect ratios of the crystals and spores were calculated. The statistical analysis of the data showed the variability of the size distribution and morphological data of the crystals produced by the analyzed strains. Furthermore, variations in spore size and shape within the same serovar were observed, indicating that, perhaps, there are still some unexplored differences between strains of this serovar, making them less identical than what was believed so far.

Automated summary

This paper reports a morphological study of crystals and spores synthesized by seven different strains of Bacillus thuringiensis, revealing variations in size distribution and morphology among the strains. The research suggests that there may be unexplored differences between strains of the same serovar, indicating that they may be less identical than previously believed.