期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 7, 期 2, 页码 462-471出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.0c01308
关键词
recombinant spider silk proteins; biomimetic materials; rheology; protein secondary structure
资金
- European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program [815357]
- Center for Innovative Medicine (CIMED) at Karolinska Institutet
- Stockholm City Council
- SFO Regen [FOR 4-1364/2019]
- Swedish Research Council [2019-01257]
- Formas [2019-00427]
- European Union [713475]
- Swedish Research Council [2019-01257] Funding Source: Swedish Research Council
- Formas [2019-00427] Funding Source: Formas
- European Research Council (ERC) [815357] Funding Source: European Research Council (ERC)
Recombinant spider silk protein NT2RepCT, when produced and spun into fibers at native-like concentrations, exhibits similar secondary structure content and viscoelastic rheological properties to native silk dope. These properties are crucial for biomimetic spinning and optimizing rheological properties could enhance the successful spinning of artificial spider silk dopes into fibers.
Recombinant spider silk has emerged as a biomaterial that can circumvent problems associated with synthetic and naturally derived polymers, while still fulfilling the potential of the native material. The artificial spider silk protein NT2RepCT can be produced and spun into fibers without the use of harsh chemicals and here we evaluate key properties of NT2RepCT dope at native-like concentrations. We show that NT2RepCT recapitulates not only the overall secondary structure content of a native silk dope but also emulates its viscoelastic rheological properties. We propose that these properties are key to biomimetic spinning and that optimization of rheological properties could facilitate successful spinning of artificial dopes into fibers.
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