期刊
STEM CELL REPORTS
卷 15, 期 6, 页码 1246-1259出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2020.11.005
关键词
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资金
- National Health and Medical Research Council (NHMRC) [APP1069830]
- Australian Research Council (ARC) Stem Cells Australia Special Initiative
- NHMRC CDF [APP1036587]
- ARC Future Fellowship [FT120100862, FT180100674]
- Silvia and Charles Viertel Senior Medical Research Fellowship
- ARC Center of Excellence program in Plant Energy Biology [CE140100008]
- HHMI International Research Scholarship
- NHMRC Investigator grant [GNT1178460]
- NHMRC ECF [APP1092280]
- State Government of Victoria
- Australian Government
- Australian Research Council [FT180100674] Funding Source: Australian Research Council
Cellular identity is ultimately dictated by the interaction of transcription factors with regulatory elements (REs) to control gene expression. Advances in epigenome profiling techniques have significantly increased our understanding of cell-specific utilization of REs. However, it remains difficult to dissect the majority of factors that interact with these REs due to the lack of appropriate techniques. Therefore, we developed TINC: TALE-mediated isolation of nuclear chromatin. Using this new method, we interrogated the protein complex formed at the Nanog promoter in embryonic stem cells (ESCs) and identified many known and previously unknown interactors, including RCOR2. Further interrogation of the role of RCOR2 in ESCs revealed its involvement in the repression of lineage genes and the fine-tuning of pluripotency genes. Consequently, using the Nanog promoter as a paradigm, we demonstrated the power of TINC to provide insight into the molecular makeup of specific transcriptional complexes at individual REs as well as into cellular identity control in general.
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