期刊
BIOLOGY OPEN
卷 10, 期 1, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/bio.054189
关键词
NAFLD; AdipoRon; FGF21; Metabolism; Hepatocyte differentiation; Hepatocyte-like cells
类别
资金
- Forschungskommission of the Medizinische Fakultat Heinrich-Heine University Dusseldorf - Medizinische Fakultat Heinrich-Heine University Dusseldorf
In the study of non-alcoholic fatty liver disease, researchers differentiated induced pluripotent stem cells into hepatocyte like cells from donors with varying disease stages, identifying distinct gene expression profiles related to the steatosis phenotype. Treatment with AdipoRon showed general influence on metabolism, transport, immune system, cell stress and signaling, although responses varied between cell lines.
Non-alcoholic fatty liver disease (NAFLD) is a multifactorial disease. Its development and progression depend on genetically predisposed susceptibility of the patient towards several 'hits' that induce fat storage first and later inflammation and fibrosis. Here, we differentiated induced pluripotent stem cells (iPSCs) derived from four distinct donors with varying disease stages into hepatocyte like cells (HLCs) and determined fat storage as well as metabolic adaptations after stimulations with oleic acid. We could recapitulate the complex networks that control lipid and glucose metabolism and we identified distinct gene expression profiles related to the steatosis phenotype of the donor. In an attempt to reverse the steatotic phenotype, cells were treated with the small molecule AdipoRon, a synthetic analogue of adiponectin. Although the responses varied between cells lines, they suggest a general influence of AdipoRon on metabolism, transport, immune system, cell stress and signalling.
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