期刊
BIOINSPIRED BIOMIMETIC AND NANOBIOMATERIALS
卷 9, 期 4, 页码 252-256出版社
ICE PUBLISHING
DOI: 10.1680/jbibn.20.00054
关键词
cell-surface interaction; poly(epsilon-caprolactone); scaffold
Lung adenosquamous carcinomas, cell line H125, with an expression of human epidermal growth factor receptor 1 (HER1), were cultured on two scaffolds of poly(epsilon-caprolactone) produced through electrospinning, one with a nanometric size and the other with a micrometric size. Cytotoxicity, adhesion and proliferation assays demonstrated the non-cytotoxicity of the scaffolds and the capacity of the cells to grow on them. Tumorlike morphology of cells, HER1 expression and the initial formation of invadopodia were detected through fluorescence microscopy. In the nanometric scaffolds, spheroid formation of cancerous cells was observed. Cells cultured in nanometric scaffolds presented higher resistance to cetuximab than those cultured in micrometric ones and also than cells of two-dimensional cultures. This work demonstrated the potentialities of poly(epsilon-caprolactone) scaffolds for lung adenosquamous carcinoma cultures and is an initial step for further studies on the growth of cancer cell spheroids on electrospun nanofibers.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据