Carbon nanotubes catalyzed UV-trigger production of hyaluronic acid from Streptococcus equi

Hyaluronic acid (HA) has great importance in biomedical applications. In this work, a novel nanoparticlebased method that stimulates the hyaluronic acid (HA) production by the bacteria Streptococcus equi subsp. Zooepidemicus has been reported. CNTs with diameters of 40-50 nm and lengths of about 20 mm were used at four different concentrations (0, 10, 25, 50, and 100 lg) to the bacteria and determined the mass of the produced HA in dependence on the exposure time under UV-irradiation. The results clearly showed that the exposure for one minute with low power UV light (254 nm) and 100 mg (CNTs) treatments steadily increased HA production from the control (0.062 g/L) to the highest value (0.992) g/L of HA. The incubation of the streptococci with CNTs led to an increase of the HA production by a factor of 4.23 after 300S exposure time under UV light, whereas the HA production was no significant enhancement under visible light. It is explained that the CNTs nanoparticle-stimulated increase of the HA production with the internalization of the nanoparticles by the bacteria since they serve as coenzymes under induced mutation by UV-irradiation. Transformation process was carried out and showed that the major protein band of Streptococcus equi was observed in the Streptococcus DH5a. RAPD analysis indicates that the amplified DNA fragments and the percentage of polymorphism was similar between Streptococcus equi and Streptococcus DH50a. The chemical structure and molecular weight of the photoproduced HA from Streptococcus equi was similar to the chemical structure of the standard sample. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.

Automated summary

This study introduced a novel nanoparticle-based method to stimulate the production of hyaluronic acid by Streptococcus equi subsp. Zooepidemicus. Results indicated that the incubation of bacteria with CNTs under UV-irradiation significantly increased HA production, with the nanoparticles serving as coenzymes to induce mutation and enhance production.