期刊
MICROMACHINES
卷 12, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/mi12010075
关键词
immunosensor; electrodeposition; Lactobacillus brevis; ionic liquid; beer; cyclic voltammetry
A novel electrochemical immunosensor was fabricated for ultra-sensitive determination of Lactobacillus brevis, with gold nanoparticles electro-deposited on the electrode surface and ionic liquid used for improving the sensor's immobilization performance. After optimization, a linear regression equation was observed between the current increment and L. brevis concentration, with a detection limit estimated at 10^3 CFU/mL.
Lactobacillus brevis is the most common bacteria that causes beer spoilage. In this work, a novel electrochemical immunosensor was fabricated for ultra-sensitive determination of L. brevis. Gold nanoparticles (AuNPs) were firstly electro-deposited on the electrode surface for enhancing the electro-conductivity and specific surface area. Ionic liquid was used for improving the immobilization performance of the immunosensor. After optimization, a linear regression equation can be observed between the increment (current) and concentration of L. brevis from 10(4) CFU/mL to 10(9) CFU/mL. The limit of detection can be estimated to be 10(3) CFU/mL.
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