期刊
MICROMACHINES
卷 12, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/mi12010053
关键词
bilayer lipid membrane; droplet interface bilayer; membrane protein; α -hemolysin; ion channel current
类别
资金
- Regional Innovation Strategy Support Program of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
This paper introduces a new method for BLM formation that does not require an organic solvent coating layer, making it suitable for directly adding chemicals to the aqueous phase compared to traditional methods.
This paper describes a method for a bilayer lipid membrane (BLM) formation using a perforated sheet along with an open chamber. Microscopic observation of the formed membrane showed a typical droplet interface bilayer. We proved that the formed membrane was a BLM based on electrical measurements of the membrane protein alpha-hemolysin, which produces nanopores in BLMs. Unlike the conventional approach for BLM formation based on the droplet contact method, this method provides aqueous surfaces with no organic solvent coating layer. Hence, this method is suitable for producing BLMs that facilitate the direct addition of chemicals into the aqueous phase.
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