期刊
JAMA OPHTHALMOLOGY
卷 139, 期 4, 页码 383-388出版社
AMER MEDICAL ASSOC
DOI: 10.1001/jamaophthalmol.2020.6339
关键词
-
资金
- Netzwerk Universitatsmedizin
This case series found the presence of SARS-CoV-2 RNA in the cornea of deceased patients with confirmed COVID-19, but did not confirm infectivity or the presence of viral structural proteins in any eye. Further research is needed to assess the rate of SARS-CoV-2 transmission via corneal transplant.
This case series investigates the presence of viral severe acute respiratory syndrome coronavirus 2 RNA in corneal discs of deceased patients with confirmed coronavirus disease 2019 and assesses viral genomic and subgenomic RNA load, possible infectivity, and histologic abnormalities. Importance Current recommendations are to avoid tissue for corneal transplant from donors with coronavirus disease 2019 (COVID-19) or those who were recently exposed to COVID-19 owing to the lack of knowledge about the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in corneal tissues. Evidence of SARS-CoV-2 in corneal tissue would seem to have clinical relevance for corneal transplant. Objectives To investigate the presence of viral SARS-CoV-2 RNA in corneal discs of deceased patients with confirmed COVID-19 and assess viral genomic and subgenomic RNA load, possible infectivity, and histologic abnormalities. Design, Setting, and Participants A case series was conducted of 11 deceased patients with COVID-19 who underwent autopsy between March 20 and May 14, 2020. Eleven corneal discs (1 corneal disc per patient) were harvested for molecular detection of viral genomic and subgenomic RNA, virus isolation, and immunohistochemistry. The SARS-CoV-2 RNA loads were compared with RNA loads in the conjunctival and throat swab samples and aqueous humor, vitreous humor, and blood samples. Main Outcomes and Measures Evidence of SARS-CoV-2 RNA in human corneas. Results This study comprised 11 patients (6 women [55%]; mean [SD] age, 68.5 [18.8] years). In 6 of 11 eyes (55%), SARS-CoV-2 genomic RNA was detected in the cornea; subgenomic RNA was present in 4 of these 6 eyes (67%). Infectivity or the presence of viral structural proteins could not be confirmed in any eye. However, patients whose corneal disc was positive for SARS-CoV-2 RNA also had positive results for SARS-CoV-2 RNA in 4 of 6 conjunctival swab samples, 1 of 3 aqueous humor samples, 3 of 5 vitreous humor samples, and 4 of 5 blood samples. Overall, conjunctival swab samples had positive results for SARS-CoV-2 RNA in 5 of 11 cases. Postmortem SARS-CoV-2 viremia was detected in 5 of 9 patients. Conclusions and Relevance Viral genomic and subgenomic RNA of SARS-CoV-2 was detected in the cornea of patients with COVID-19 viremia. The risk of COVID-19 infection via corneal transplant is low even in donors with SARS-CoV-2 viremia, but further research is necessary to assess the rate of SARS-CoV-2 transmission via corneal transplant. Question Is severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present in the human cornea? Findings In this case series carried out in a tertiary care facility, SARS-CoV-2 genomic RNA was detected in the cornea of 6 of 11 eyes (55%) of patients with viremic coronavirus disease 2019; subgenomic SARS-CoV-2 RNA was present in 4 of these 6 eyes (67%). Infectivity or presence of viral structural proteins could not be confirmed in any eye. Meaning This report demonstrates the presence of viral genomic and subgenomic RNA of SARS-CoV-2 in the human cornea.
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