期刊
JOURNAL OF NANOBIOTECHNOLOGY
卷 19, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12951-020-00757-5
关键词
Hair growth; Nanovesicles; Extracellular vesicles
资金
- National Natural Science Foundation of China [81972364, 81602787]
- Jiangsu Natural Science Foundation [BK20170107, BK20180165]
- Science and Technology Development Foundation of Nanjing Medical University [2017NJMUZD109]
- Scientific Research Project of Wuxi Municipal Health Commission [MS 201821]
- Wuxi Science and Technology Development Fund [N20202002]
The study found that ReN-NV promotes DPC proliferation and accelerates HF cycling transition in a mouse model. This acceleration is achieved through the activation of the Wnt/beta-catenin signaling pathway.
Background Accumulating evidence shows that mesenchymal stem cell-derived extracellular vesicles (EVs) hold great promise to promote hair growth. However, large-scale production of EVs is still a challenge. Recently, exosome-mimetic nanovesicles (NV) prepared by extruding cells have emerged as an alternative strategy for clinical-scale production. Here, ReNcell VM (ReN) cells, a neural progenitor cell line was serially extruded to produce NV. Results ReN-NV were found to promote dermal papilla cell (DPC) proliferation. In addition, in a mouse model of depilation-induced hair regeneration, ReN-NV were injected subcutaneously, resulting in an acceleration of hair follicle (HF) cycling transition at the site. The underlying mechanism was indicated to be the activation of Wnt/beta-catenin signaling pathway. Furthermore, miR-100 was revealed to be abundant in ReN-NV and significantly up-regulated in DPCs receiving ReN-NV treatment. miR-100 inhibition verified its important role in ReN-NV-induced beta-catenin signaling activation. Conclusion These results provide an alternative agent to EVs and suggest a strategy for hair growth therapy.
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