4.6 Article

Extracellular Vesicles Derived From Talaromyces marneffei Yeasts Mediate Inflammatory Response in Macrophage Cells by Bioactive Protein Components

期刊

FRONTIERS IN MICROBIOLOGY
卷 11, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2020.603183

关键词

Talaromyces marneffei; extracellular vesicles; macrophage cells; inflammatory response; proteomics

资金

  1. National Natural Science Foundation of China [81601819, 81772244]
  2. Outstanding Youths Development Scheme of Nanfang Hospital, Southern Medical University [2016J013]
  3. Medical Science and Technology Research Foundation of Guangdong Province [A2016280]
  4. President Foundation of Nanfang Hospital, Southern Medical University [2019C003]
  5. China Postdoctoral Science Foundation [2019M662990]

向作者/读者索取更多资源

TM-derived EVs are a heterogeneous group of nanosized membrane vesicles that can be taken up by macrophages, leading to increased inflammatory response and upregulation of co-stimulatory molecules in macrophages. The proteins in EVs play a crucial role in regulating the function of RAW 264.7 macrophage cells.
Extracellular vesicles (EVs) loaded with proteins, nucleic acids, membrane lipids, and other virulence factors could participate in pathogenic processes in some fungi such as Cryptococcus neoformans and Candida albicans. However, the specific characteristics of EVs derived from Talaromyces marneffei (TM) still have not been figured out yet. In the present study, it has been observed that TM-derived EVs were a heterogeneous group of nanosized membrane vesicles (30-300 nm) under nanoparticle tracking analysis and transmission electron microscopy. The DiI-labeled EVs could be taken up by RAW 264.7 macrophage cells. Incubation of EVs with macrophages would result in increased expression levels of reactive oxygen species, nitric oxide, and some inflammatory factors including interleukin-1 beta, interleukin-6, interleukin-10, and tumor necrosis factor. Furthermore, the expression of co-stimulatory molecules (CD80, CD86, and MHC-II) was also increased in macrophages stimulated with EVs. The level of inflammatory factors secreted by macrophages showed a significant decrease when EVs were hydrolyzed by protease, while that of DNA and RNA hydrolase treatment remained unchanged. Subsequently, some virulence factors in EVs including heat shock protein, mannoprotein 1, and peroxidase were determined by liquid chromatography-tandem mass spectrometry. Taken together, our results indicated that the TM-derived EVs could mediate inflammatory response and its protein would play a key role in regulating the function of RAW 264.7 macrophage cells.

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