Investigation of Physicochemical Indices and Microbial Communities in Termite Fungus-Combs

Termitomyces species are wild edible mushrooms that possess high nutritional value and a wide range of medicinal properties. However, the cultivation of these mushrooms is very difficult because of their symbiotic association with termites. In this study, we aimed to examine the differences in physicochemical indices and microbial communities between combs with Termitomyces basidiomes (CF) and combs without Termitomyces basidiomes (CNF). High-performance liquid chromatography (HPLC), inductively coupled plasma optical emission spectrometry (ICP-OES), gas chromatography equipped with a flame ionization detector (GC-FID), some commercial kits, high-throughput sequencing of the 16s RNA, and internal transcribed spacer (ITS) were used. Humidity, pH, and elements, i.e., Al, Ba, Fe, Mn, Ni, S, Ca, and Mg were higher while amino acids particularly alanine, tyrosine, and isoleucine were lower in CF as compared to CNF. The average contents of fatty acids were not significantly different between the two comb categories. The bacterial genera Alistipes, Burkholderia, Sediminibacterium, and Thermus were dominant in all combs. Brevibacterium, Brevundimonas, and Sediminibacterium were significantly more abundant in CF. Basidiomycota and Ascomycota were also identified in combs. Termitomyces clypeatus, Termitomyces sp. Group3, and Termitomyces sp. were the most dominant species in combs. However, any single Termitomyces species was abundantly present in an individual comb.

Automated summary

Termitomyces species are wild edible mushrooms that are difficult to cultivate due to their symbiotic association with termites. This study examined the differences in physicochemical indices and microbial communities between combs with and without Termitomyces basidiomes. Fatty acid contents were similar in both comb categories, while bacterial genera Alistipes, Burkholderia, Sediminibacterium, and Thermus were dominant in all combs, with other genera more abundant in combs with Termitomyces basidiomes. Basidiomycota and Ascomycota were also identified in combs, with various Termitomyces species present but not abundantly in individual combs.