期刊
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷 10, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2020.594778
关键词
choledocholithiasis; bile; biliary microbiota; endoscopic sphincterotomy; amplicon sequencing
资金
- Natural Science Foundation of Zhejiang Province [LY17H030003]
- National Natural Science Foundation of China [31701158, 31870079, 81602325]
- Zhejiang Medical and Health Science and Technology Plan [WKJ-ZJ-2136, 2019RC068]
- Hangzhou Medical and Health Science and Technology Plan [2016ZD01, OO20190610, A20200174]
- Beijing Nova Program [Z181100006218114]
The study revealed that a history of invasive endoscopic sphincterotomy (EST) significantly influences the biliary microbiota, leading to a marked shift in the composition of biliary bacteria. The bacterial genus Pyramidobacter was specifically associated with a history of EST.
The biliary microbiota is related to the pathogenesis of human bile duct stones. However, the extent to which a history of invasive endoscopic sphincterotomy (EST) affects the biliary bacterial community remains largely unknown. We collected bile samples from the common bile duct of 100 choledocholithiasis patients. We performed 16S rRNA sequencing to investigate and compare the biliary microbial community. The patients without antibiotic treatment (AT) were grouped into three clusters based on their biliary microbial compositions. The patients with a history of EST were significantly enriched in one cluster mainly consisting of gastrointestinal bacteria compared with the other two clusters consisting of oral and environmental bacteria. The beta-diversities of patients with and without EST were also significantly different, whereas the alpha-diversities were comparable. The only significantly enriched bacterial genus associated with a history of EST was Pyramidobacter, while eight other genera were significantly decreased. For patients with AT, seven of these genera maintained their association with EST, including Pyramidobacter. However, after AT, the difference in beta-diversities was diminished. EST induced a marked shift in the biliary microbial composition. A cluster of biliary bacteria was associated with a history of EST, and Pyramidobacter was specific to EST.
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