Antibacterial Activity of N,O-Acylated Chitosan Derivative

The antibacterial activity of N,O-acylated chitosan derivative with linoleic acid (CH_LA) was tested by disc and well diffusion, agar impregnation and microdilution methods against Staphylococcus aureus, Escherichia coli and Helicobacter pylori strains. Hydrophobically modified chitosan (HMC) was expected to exhibit enhanced antibacterial activity and specific mucin interactions. Although diffusion tests have not indicated the antibacterial potential of chitosan (CH) or CH_LA, the results of the microdilution method demonstrated that tested polymers significantly reduced the amount of living bacteria cells in different concentrations depending on the microorganism. Additionally, CH_LA was characterized by enhanced antibacterial activity compared to CH, which may suggest a different mechanism of interaction with S. aureus and H. pylori. Furthermore, the UV-VIS analysis revealed that the amphiphilic character of derivative led to strong CH_LA-mucin interactions. The study proved the high potential of CH_LA in antibacterial applications, especially for the gastrointestinal tract.

Automated summary

The antibacterial activity of N,O-acylated chitosan derivative with linoleic acid was tested against Staphylococcus aureus, Escherichia coli, and Helicobacter pylori strains using various methods. The results showed that the derivative exhibited enhanced antibacterial activity and specific mucin interactions, especially against S. aureus and H. pylori. This study highlights the high potential of the derivative in antibacterial applications, particularly in the gastrointestinal tract.