期刊
JOURNAL OF GINSENG RESEARCH
卷 45, 期 2, 页码 354-360出版社
KOREAN SOC GINSENG
DOI: 10.1016/j.jgr.2020.12.003
关键词
protopanaxatriol; skin protection; NF-kappa B; AP-1; CREB
资金
- Konkuk University, South Korea
PPT enhances skin barrier function and hydration by promoting the expression of filaggrin, transglutaminase, claudin, hyaluronic acid synthase, and activating Src/AKT/NF-kappa B and MAPK signaling pathways.
Background: Protopanaxatriol (PPT) is a secondary intestinal metabolite of ginsenoside in ginseng. Although the effects of PPT have been reported in various diseases including cancer, diabetes and inflammatory diseases, the skin protective effects of PPT are poorly understood. Methods: HaCaT cells were treated with PPT in a dose-dependent manner. mRNA and protein levels which related to skin barrier and hydration were detected compared with retinol. Luciferase assay was performed to explore the relative signaling pathway. Western blot was conducted to confirm these pathways and excavated further signals. Results: PPT enhanced the expression of filaggrin (FLG), transglutaminase (TGM)-1, claudin, occludin and hyaluronic acid synthase (HAS) -1, -2 and -3. The mRNA expression levels of FLG, TGM-1, HAS-1 and HAS-2 were suppressed under NF-kappa B inhibition. PPT significantly augmented NF-kappa B-luc activity and upregulated Src/AKT/NF-kappa B signaling. In addition, PPT also increased phosphorylation of the mitogen-activated protein kinases (MAPKs) ERK, JNK and p38 and upstream MAPK activators (MEK and MKK). Furthermore, transcriptional activity of AP-1 and CREB, which are downstream signaling targets of MAPK, was enhanced by PPT. Conclusion: PPT improves skin barrier function and hydration through Src/AKT/NF-kappa B and MAPK signaling. Therefore, PPT may be a valuable component for cosmetics or treating skin disorders. (C) 2020 The Korean Society of Ginseng. Publishing services by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据