Electron Microscopic Reconstruction of Neural Circuitry in the Cochlea

Recent studies reveal great diversity in the structure, function, and efferent innervation of afferent synaptic connections between the cochlear inner hair cells (IHCs) and spiral ganglion neurons (SGNs), which likely enables audition to process a wide range of sound pressures. By performing an extensive electron microscopic (EM) reconstruction of the neural circuitry in the mature mouse organ of Corti, we demonstrate that afferent SGN dendrites differ in abundance and composition of efferent innervation in a manner dependent on their afferent synaptic connectivity with IHCs. SGNs that sample glutamate release from several presynaptic ribbons receive more efferent innervation from lateral olivocochlear projections than those driven by a single ribbon. Next to the prevailing unbranched SGN dendrites, we found branched SGN dendrites that can contact several ribbons of 1-2 IHCs. Unexpectedly, medial olivocochlear neurons provide efferent innervation of SGN dendrites, preferring those forming single-ribbon, pillar-side synapses. We propose a fine-tuning of afferent and efferent SGN innervation.

Automated summary

Recent studies have shown a great diversity in the afferent synaptic connections between IHCs and SGNs, with varying abundance and composition of efferent innervation depending on the connectivity of the afferent SGN dendrites with IHCs. Different levels of efferent innervation are provided to SGNs based on the number and source of stimuli received, and branched SGN dendrites and medial olivocochlear neurons were also found to play a role in efferent innervation of SGN dendrites.